The first division of HeLa × chick erythrocyte heterokaryons
Identifieur interne : 000983 ( Main/Exploration ); précédent : 000982; suivant : 000984The first division of HeLa × chick erythrocyte heterokaryons
Auteurs : R. Appels [Suède, Australie] ; P. B. Bell [Suède, États-Unis] ; N. R. Ringertz [Suède]Source :
- Experimental Cell Research [ 0014-4827 ] ; 1975.
Abstract
Chick erythrocyte nuclei introduced into HeLa cells by Sendai virus induced cell fusion may be transferred, intact to daughter cells, when the heterokaryons undergo mitosis in the first 20–30 h post-fusion. The evidence presented is based on (1) population analyses of heterokaryon cultures at various times post-fusion; (2) analysis of mitotic heterokaryons in fixed cell preparations; and (3) single cell analysis by time-lapse cinematography of living cells from the time of fusion until division. The data indicated that transfer of intact chick nuclei to daughter cells from a heterokaryon was a common event. Premature chromosome condensation and incorporation of chick and human chromosomes into the same post-mitotic nuclei may have occurred in some cases where the chick nuclei disappeared. In those cases where the erythrocyte nuclei were transferred to one of the daughter cells of a dividing heterokaryon, the chick nucleus was found to undergo very rapid swelling during early G 1 phase, i.e. during the time when the HeLa nucleus was transformed from a telophase nucleus to a G 1 nucleus. This indicated that the chick erythrocyte nuclei may respond to changes in the condition of the cytoplasm which are reflected in the swelling of telophase HeLa nuclei into early G 1 nuclei.
Url:
DOI: 10.1016/0014-4827(75)90639-4
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Appels</name><affiliation wicri:level="1"><country xml:lang="fr">Suède</country><wicri:regionArea>Institute for Medical Cell Research and Genetics, Medical Nobel Institute, Karolinska Institutet, S-104 01 Stockholm 60</wicri:regionArea><wicri:noRegion>S-104 01 Stockholm 60</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Pathology and the Wallenberg Laboratory, University of Uppsala, S-752 47 Uppsala</wicri:regionArea><wicri:noRegion>S-752 47 Uppsala</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Australie</country><wicri:regionArea>1 Present address: Division of Plant Industry, CSIRO, P.O. Box 1600 Canberra, ACT</wicri:regionArea><wicri:noRegion>ACT</wicri:noRegion></affiliation></author><author><name sortKey="Bell, P B" sort="Bell, P B" uniqKey="Bell P" first="P. B." last="Bell">P. B. 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The evidence presented is based on (1) population analyses of heterokaryon cultures at various times post-fusion; (2) analysis of mitotic heterokaryons in fixed cell preparations; and (3) single cell analysis by time-lapse cinematography of living cells from the time of fusion until division. The data indicated that transfer of intact chick nuclei to daughter cells from a heterokaryon was a common event. Premature chromosome condensation and incorporation of chick and human chromosomes into the same post-mitotic nuclei may have occurred in some cases where the chick nuclei disappeared. In those cases where the erythrocyte nuclei were transferred to one of the daughter cells of a dividing heterokaryon, the chick nucleus was found to undergo very rapid swelling during early G 1 phase, i.e. during the time when the HeLa nucleus was transformed from a telophase nucleus to a G 1 nucleus. This indicated that the chick erythrocyte nuclei may respond to changes in the condition of the cytoplasm which are reflected in the swelling of telophase HeLa nuclei into early G 1 nuclei.</div></front></TEI><affiliations><list><country><li>Australie</li><li>Suède</li><li>États-Unis</li></country></list><tree><country name="Suède"><noRegion><name sortKey="Appels, R" sort="Appels, R" uniqKey="Appels R" first="R." last="Appels">R. Appels</name></noRegion><name sortKey="Appels, R" sort="Appels, R" uniqKey="Appels R" first="R." last="Appels">R. Appels</name><name sortKey="Bell, P B" sort="Bell, P B" uniqKey="Bell P" first="P. B." last="Bell">P. B. Bell</name><name sortKey="Bell, P B" sort="Bell, P B" uniqKey="Bell P" first="P. B." last="Bell">P. B. Bell</name><name sortKey="Ringertz, N R" sort="Ringertz, N R" uniqKey="Ringertz N" first="N. R." last="Ringertz">N. R. Ringertz</name><name sortKey="Ringertz, N R" sort="Ringertz, N R" uniqKey="Ringertz N" first="N. R." last="Ringertz">N. R. Ringertz</name></country><country name="Australie"><noRegion><name sortKey="Appels, R" sort="Appels, R" uniqKey="Appels R" first="R." last="Appels">R. Appels</name></noRegion></country><country name="États-Unis"><noRegion><name sortKey="Bell, P B" sort="Bell, P B" uniqKey="Bell P" first="P. B." last="Bell">P. B. Bell</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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